RE: [Histonet] Pig endothelium....again

From:"Swain, Frances L"

Hi Allistar:  I fix my air-dried ( the tech that cuts the frozens air dries
for 60 minutes at room temp prior to placing in a plastic box and freezing
the sections.  When I get ready to stain them for IHC or regular staining I
remove the sections that I need and place them immediately in cold 100%
Acetone for 20 minutes.  I then proceed with my protocol. 


Frances L. Swain HT(ASCP) A. A. S.

Special Procedures Technician

Department of Orthopaedic Surgery

Center for Orthopaedic Research

Barton Research Building 2R28

4301 West Markham Street

Little Rock AR 72205

(501) 686-8739 PHONE

(501) 686-8987 FAX email


-----Original Message-----
[] On Behalf Of Alistair
Sent: Thursday, October 16, 2008 3:36 PM
Subject: [Histonet] Pig endothelium....again


Dear Histonetters,


You may recall my earlier post on rings of pig aorta.  I have managed to

keep them alive quite nicely with DMEM - many thanks for the tips.


Unfortunately, I am still struggling to stain for endothelial adhesion



I have read and received various different opinions on fixation after I have

made my cryostat sections.  Should the slides be fixed immediately, or air

dried first? Acetone or formalin?


Any tips would be gratefully received.


Thank You,






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