RE: [Histonet] Oil Red O despair

From:"Tony Henwood"

One of the main conditions that may prevent an adequate Oil Red O stain
is the saturation of the dye. It can take one or more weeks for the Oil
Red O dye to reach saturation in the solvent.

We have two bottles of Oil Red O solution prepared. We always prepare
another batch when the first is emptied so that one is "ripening" while
the older solution is being used. (Oh gee - did that make sense?)


Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager & Senior Scientist
Tel: 612 9845 3306
Fax: 612 9845 3318
the children's hospital at westmead 
Cnr Hawkesbury Road and Hainsworth Street, Westmead 
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA 

-----Original Message-----
[] On Behalf Of Michele
Sent: Tuesday, 18 November 2008 4:57 AM
Subject: [Histonet] Oil Red O despair

I'm wondering what are the pros (or cons) of using the propylene glycol
version of Oil Red O over the isopropanol method. Is one more suited to
a specific application? 

I'm trying to stain a frozen section of liver, which one would suspect
would be loaded with fat, and have thus far been unsuccessful using the
propylene glycol Oil Red O.

Is there something obvious that I'm missing here? I'm new to
cryosectioning...perhaps I did something wrong in the cryostat. I fixed
my sections in NBF before staining.

Please help. I'm feeling like a pathetic excuse for a histotech. Any
advice is greatly appreciated.

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