RE: [Histonet] IHC antibody Expiration question for CAP

From:"Poteete, Jacquie A."



I agree, it is a very ridiculous rule, and I don't like having to
eliminate perfectly good antibodies or reagents.  When you look at the
CAP guidelines, there are lots of ridiculous rules, in my humble
opinion.  Let the flaming begin!

Jacquie Poteete MT(ASCP)QIHC
Lead Technologist, IHC Lab
Saint Francis Hospital
Tulsa, OK 

-----Original Message-----
From: Marshall Terry Dr, Consultant Histopathologist
[mailto:Terry.Marshall@rothgen.nhs.uk] 
Sent: Friday, October 10, 2008 10:07 AM
To: Poteete, Jacquie A.; themagoos@rushmore.com;
histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] IHC antibody Expiration question for CAP


What a ridiculous rule.
If the antibody works on a control, THAT is the test for whether it is
useful or not, not an arbitrary date printed on the tube.

Terry 

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Poteete,
Jacquie A.
Sent: 10 October 2008 15:32
To: themagoos@rushmore.com; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] IHC antibody Expiration question for CAP

We are not permitted to use anything that is expired, no matter what it
is.  Yes, the lab takes a major hit because of this regulation.  I send
my expired antibodies to Patsy Ruegg so she can use them for
experimental purposes. It isn't a total loss, just a very expensive one.
Thanks for the reminder, because I need to send her another bunch.

Jacquie Poteete MT(ASCP)QIHC
Lead Technologist, IHC Lab
Saint Francis Hospital
Tulsa, OK

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jason
and Heather
Sent: Friday, October 10, 2008 9:22 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] IHC antibody Expiration question for CAP


CAP question ANP.22432 states: Are all immunohistochemical reagents used
within their indicated expiration dates? 

I am wondering what labs across the country are doing to answer this
question. IHC antibodies are very expensive and can be validated on
every run that is made. Some of these concentrated antibodies are
diluted out so far that only a very small portion of them are used
before they expire. Are some labs still using outdated antibodies? If so
how do you get away with it when you are inspected by CAP? Or are you
paying the high prices to throw out the majority of your antibodies so
you can answer this question yes? 

I thank you in advance to your response.

Jason McGough HT(ASCP)
Account Representative - Anatomic Pathology Clinical Laboratory of the
Black Hills 2805 5th Street Suite 210 Rapid City, SD 57701 605-343-2267
Ext 127 605-718-3779 (Fax) jmcgough@clinlab.com


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