RE: [Histonet] Histology and immuonhistochemistry of Drosophilaeyesections (OT)

From:"Jo Dee Fish"



Hi Pam,
I have worked with the little guys for a while now.  The best way is to
pre-embed in agar or histogel (although I have had a lot of bad luck with
the histogel getting "crispy" in our processor).  Put a small amount of agar
in a small mold, like the tissue tek plastic molds, then align the flies in
the orientation that you would like.  Then just process the entire block of
agar into paraffin and embed.  They cut beautifully.
Good luck,
Jo Dee 


~~Jo Dee Fish~~
Senior Research Technologist
The J. David Gladstone Institutes
Co-manager Histology and Microscopy Core
 
Telephone: (415) 734-2567
Fax: (415) 355-0824
E-mail: jfish@gladstone.ucsf.edu
 
Mailing address:
The J. David Gladstone Institutes
1650 Owens Street
San Francisco, CA 94158

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Peggy Bisher
Sent: Tuesday, October 28, 2008 8:34 AM
To: Ingles Claire; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Histology and immuonhistochemistry of
Drosophilaeyesections (OT)

I have students here who have been doing cryo-sectioning of Drosophila. No
one, has yet tried it in paraffin. Since they are very tiny flies, we have a
contraption that allows us to orient the flies so that their heads are all
in a row and we just cut a whole bunch at once. It looks like a slide with a
long groove in it - just wide enough to fit their heads above it and the
bodies hang down below. It seems to work just fine.

Cheers, Peggy


Margaret E. Bisher
Electron Microscopy & Histology Core Facility Manager Department of
Molecular Biology Princeton University Moffett Laboratory, Room 113
Princeton, New Jersey
Office: (609) 258-7026
Fax: (609) 258-8468
mbisher@princeton.edu




On 10/27/08 9:46 PM, "Ingles Claire"  wrote:

>  
> Fly's eyes? What about bee's knees?
> Sorry it's almost 9pm. I'm fizzling out.
> Claire :o
> 
> ________________________________
> 
> From: histonet-bounces@lists.utsouthwestern.edu on behalf of pam 
> johnson
> Sent: Mon 10/27/2008 4:39 PM
> To: histonet@lists.utsouthwestern.edu
> Subject: [Histonet] Histology and immuonhistochemistry of Drosophila 
> eyesections
> 
> 
> 
> 
> I have been approached by an investigator?who wants us to do some work 
> on fly eyes. Has anyone had any experience with fly eyes in paraffin? 
> If so can you please share your protocols on processing and cutting. 
> Aren't they very crunchy? Do you process the whole head?
> 
> ?
> 
> Any information would be greatly appreciated.
> 
> Thanks,
> 
> 
> Pam Johnson, BS, HT (ASCP)
> Lab Manager
> Veterinary Pathology Core Lab
> St. Jude Children's Research Hospital
> 262 Danny Thomas Place
> Memphis, TN? 38105-3678
> Office - 901-595-3355
> 
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> 
> 
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