RE: [Histonet] Fatty tissue

From:"Tony Henwood"

"anecdotal" but consistent


Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) 
Laboratory Manager & Senior Scientist 
Tel: 612 9845 3306 
Fax: 612 9845 3318 
the children's hospital at westmead
Cnr Hawkesbury Road and Hainsworth Street, Westmead
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA 

	-----Original Message-----
	From: Rene J Buesa [] 
	Sent: Saturday, 8 November 2008 12:56 AM
	To: Tony Henwood; Webb, Dorothy L;; Luck, Greg D.
	Subject: RE: [Histonet] Fatty tissue
The MW irradiation will NOT accelerate formalin cross linking; essentially MW irradiation will have a "cooking" effect on tissue, that is why the "6-48" rule was issued by ASCO-CAP
After MW irradiation the tissues that go into dehydration will be only partially fixed with formalin and will end being fixed by the alcohols in a "2 fixation" process with unknown consequences for the procedures that follow.The anecdotal "successful" use of MW for tissue fixation are just that, anecdotal,
René J.

--- On Thu, 11/6/08, Luck, Greg D.  wrote:

	From: Luck, Greg D. 
	Subject: RE: [Histonet] Fatty tissue
	To: "Tony Henwood" , "Webb, Dorothy L" ,
	Date: Thursday, November 6, 2008, 7:14 PM
	Are people microwave fixing breast tissues?  Just wondering as/does this
	fly in the face of the "6-48" hour fixation requirement for any
	tissue that is to have Her2-neu testing?
	Thanks, Greg

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