The MW irradiation will NOT accelerate formalin cross linking; essentially MW irradiation will have a "cooking" effect on tissue, that is why the "6-48" rule was issued by ASCO-CAP
After MW irradiation the tissues that go into dehydration will be only partially fixed with formalin and will end being fixed by the alcohols in a "2 fixation" process with unknown consequences for the procedures that follow.The anecdotal "successful" use of MW for tissue fixation are just that, anecdotal,
--- On Thu, 11/6/08, Luck, Greg D. wrote:
From: Luck, Greg D.
Subject: RE: [Histonet] Fatty tissue
To: "Tony Henwood" , "Webb, Dorothy L" , email@example.com
Date: Thursday, November 6, 2008, 7:14 PM
Are people microwave fixing breast tissues? Just wondering as/does this
fly in the face of the "6-48" hour fixation requirement for any
tissue that is to have Her2-neu testing?
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