The MW irradiation will NOT accelerate formalin cross linking; essentially MW irradiation will have a "cooking" effect on tissue, that is why the "6-48" rule was issued by ASCO-CAP
After MW irradiation the tissues that go into dehydration will be only partially fixed with formalin and will end being fixed by the alcohols in a "2 fixation" process with unknown consequences for the procedures that follow.The anecdotal "successful" use of MW for tissue fixation are just that, anecdotal,
René J.

--- On Thu, 11/6/08, Luck, Greg D.  wrote:

From: Luck, Greg D. 
Subject: RE: [Histonet] Fatty tissue
To: "Tony Henwood" , "Webb, Dorothy L" , histonet@lists.utsouthwestern.edu
Date: Thursday, November 6, 2008, 7:14 PM

Hello,
Are people microwave fixing breast tissues?  Just wondering as/does this
fly in the face of the "6-48" hour fixation requirement for any
breast
tissue that is to have Her2-neu testing?
Thanks, Greg

      
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet