RE: [Histonet] Fatty tissue

From:"Luck, Greg D."



Hello,
Are people microwave fixing breast tissues?  Just wondering as/does this
fly in the face of the "6-48" hour fixation requirement for any breast
tissue that is to have Her2-neu testing?
Thanks, Greg

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tony
Henwood
Sent: Wednesday, November 05, 2008 2:43 PM
To: Webb, Dorothy L; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Fatty tissue

I would suggest microwaving the tissue blocks in formalin for at least 1
hour (longer is better) up to a temperature of 45oC (you might even
consider a higher temp maybe up to 55oC??)

We use this with our placenta blocks and the results were dramatically
improved (35% poorly processed to less than 1% after microwaving).

We have a Mega TT Microwave oven 

Regards

Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory
Manager & Senior Scientist
Tel: 612 9845 3306
Fax: 612 9845 3318
the children's hospital at westmead
Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001,
Westmead NSW 2145, AUSTRALIA 




-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Webb,
Dorothy L
Sent: Thursday, 6 November 2008 7:22 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Fatty tissue


I would like to know how other labs are processing "fatty" tissue,
especially breast for optimal processing?  We have been doing a few
"reprocessing" of these specimens and my pathologist feels this should
not be happening!  Yes, the PA's are, at times, cutting the sections
larger than 3 mm thick, but, at times the tumor is ingrained in the
fatty area and they need to obtain as much of the perimeter as they can.
Does anyone agitate or heat fixate their specimens prior to processing?
Would appreciate any feedback on this age old dilemma. Also, I only
heard back from one person on the recycling issue.  Any input would be
appreciated as to savings incurred and a still vs. recycler.
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