RE: [Histonet] Expiration dates

From:"Lee & Peggy Wenk"



Except here in the US, where we have federal laws that prohibit using
expired reagents. Enforced by laboratory accrediting agencies. 

Peggy A. Wenk, HTL(ASCP)SLS
Beaumont Hospitals
Royal Oak, MI 48073

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Piero Nelva
Sent: Wednesday, October 15, 2008 3:27 AM
To: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Expiration dates

Hi Amy

I use VMS Benchmark XT and when the antibody has expired I "create" a new
lot number by putting (EXP1) at the end of it and adding one year to the
expiration date.  THen add the antibody dilution to the prepkit using the
"Refill" option rather than the "Partial" option.  As long as you're using
appropriate controls with your staining runs, there is no problem extending
the life of the antibody.

Regards

Piero Nelva
Anatomical pathology
Monash Medical Centre
Victoria
Australia





Hello all,



My supervisor wanted me to ask everyone who's posting about the expiration
dates of antibodies:  How can you use expired antibodies on a stainer such
as a Benchmark-which will not allow you to start the run due to expiration
dates on the containers?





Thanks!!



Amy Senn

Camp Hill, PA



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