Floating the trimmed/faced block on the waterbath for 2 minutes helps.
Then put them on wet ice to cool.
Regards, Laurie.
Mr. Laurie REILLY
Histopathology
School of Veterinary and Biomedical Sciences
James Cook University
Townsville Qld. 4811
Australia.
Phone 07 4781 4468
-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Piero Nelva
Sent: Friday, 3 October 2008 7:16 PM
To: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Does anyone have any ideas on the use of softener
ondecal blocks. Does this help 'soften' them? If so how?
Hi Sarah
I don't use a softener as such, but have had good results putting trimmed
blocks on a water soaked tissue on the bench for 30 min or so just before
cooling and sectioning. I find it is more effective than an ice bath at
softening tissues.
REgards
Piero Nelva
Anatomical Pathology
Monash Medical Centre
Australia
----- Original Message -----
From: "SARAH REEVES"
To:
Sent: Friday, October 03, 2008 12:34 AM
Subject: [Histonet] Does anyone have any ideas on the use of softener on
decal blocks. Does this help 'soften' them? If so how?
Does anyone have any ideas on the use of softener on decal blocks. Does this
help 'soften' them? If so how?
Thanks in advance
Sarah
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