RE: [Histonet] C4d question (not CD4)

From:"Houston, Ronald"

That's for CD4 not C4d

Ronnie Houston
Anatomic Pathology Manager
Nationwide Children's Hospital
Columbus OH 43205
(614) 722 5450
-----Original Message-----
[] On Behalf Of Jodie
Sent: Wednesday, November 19, 2008 9:34 AM
To: Richard Cartun; Histonet
Subject: RE: [Histonet] C4d question (not CD4)

Biocare has an IVD predilute that is very good.  (PM153AA)  We use it on
our Benchmark XT.=0D
Jodie Robertson, HT (ASCP) QIHC 
Pathology Sciences Medical Group
Chico, CA 
-----Original Message-----
[] On Behalf Of Richard
Sent: Tuesday, November 18, 2008 3:16 PM
To: Histonet
Subject: [Histonet] C4d question (not CD4)

We have been using an "RUO"-labeled anti-C4d antibody on certain renal
transplant cases for the immunohistochemical detection of humoral
rejection for the past several years.  We have now been asked by our
Transplant team to do this test routinely on all transplant kidney
biopsies (which is another issue).

Is anyone using an "IVD"-labeled antibody for C4d detection by IHC?
Although we get excellent results with this antibody on formalin-fixed,
paraffin-embedded tissue, I would prefer to be using an "IVD"-labeled
antibody (if one exists).  Thanks.


Richard W. Cartun, Ph.D.
Director, Histology & Immunopathology
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 545-1596
(860) 545-0174 Fax

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