RE: [Histonet] Any experience with the Mohs "cryo embedder"

From:"Mickie Johnson"

Hi Mark,

Thanks for your input on how the Leica CM 1100 is working for you. Regarding
the best way to embed tissues for Mohs. Yes, the slide method is the most
accurate to get the tissue flat as long as the epidermis is relaxed so it
lays down on the glass too. Watch out for trapped air bubbles under the
specimen. I smear a thin coat of O.C.T. on the slide and this helps hold the
epi down during embedding. Does the Leica 1100 allow you to reposition the
block face parallel to the knife when you embed? That is important so that
you don't cut any more tissue away than necessary to get a full section of
epidermis and deep margin. There is also a device which is very helpful in
embedding Mohs specimens to minimize the need to line up the block face to
the knife. Many labs use it. I carry one where ever I go to help me embed
fast and accurately. If you would like to know more give me a call. Good

Best Regards,
Mickie Johnson, B.S., HTL(ASCP)
Mohs Histology Consulting Services, LLC
  & Mohs Lab Staffing
2507 S. Manito Blvd.
Spokane, WA 99203
FAX   509-624-3926
Web: & 
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-----Original Message-----
[] On Behalf Of Mark Tarango
Sent: Thursday, October 09, 2008 12:45 PM
To: Ingles Claire
Subject: Re: [Histonet] Any experience with the Mohs "cryo embedder"

Does anyone else freeze the tissue to a glass slide to get it embedded
flat?  That's how I've been show and it seems to work well.

I've used plastic embedding molds too, but they don't always give me a flat

On Thu, Oct 9, 2008 at 12:02 PM, Ingles Claire  wrote:

> Sandra:
> Who is selling this, and how does it work? I have been testing one type of
> embedder, but I know there are several out there.
> Claire
> ________________________________
> From: on behalf of Esparza,
> Sandra
> Sent: Thu 10/9/2008 11:20 AM
> To:
> Subject: [Histonet] Any experience with the Mohs "cryo embedder"
> Hi Everyone,
> I am new to the Mohs technique for dermatology specimens and am trying
> to find out if anyone has used the Cryo Embedder system.  It sound great
> but I don't want to order it until I'm sure that it is user friendly.
> Any help with the Mohs technique would be greatly appreciated.   Thanks,
> Sandra HT (ASCP)
> Dell Children's Hospital
> Austin, Texas
> _______________________________________________
> Histonet mailing list
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> Histonet mailing list
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