RE: [Histonet] Antibody protocol

From:"Weber, Susan (VHACLE)"

We are using the ultraView DAB from Ventana and the same HSA from Cell
Marque. Here is the protocol we are using. Depar as usual; CC1 Standard;
HSA incubation at 37C for 32 min; Counterstain Heme 4 min; Post
Counterstain Bluing for 4 min. we have found it to be a reliable stain
and very clean and crisp. 

Susan M Weber HT(ASCP)
Histology Supervisor
Louis Stokes Cleveland VA Medical Center
10701 East Blvd
Cleveland, Ohio 44106
(216) 791-3800 X6154
-----Original Message-----
[] On Behalf Of Jesus
Sent: Tuesday, November 18, 2008 12:37 PM
Subject: [Histonet] Antibody protocol

Needing some help with the staining protocol for  HSA (Hepatocyte
Specific Antigen) clone OCH1E5, made by Cell marquee to be ran on the
Ventana LT/Benchmark platform.. Would anyone be willing to share
protocol information with us, as well as staining methodology.  Thank
you for the help.  


Jesus A Ellin  HT/PA  ASCP

Department of Pathology/Histology

Yuma Regional Medical Center

2400 South Ave A

Yuma, AZ  85364 - 7170

( Office:  (928) 336-1743

(    Fax:  (928) 336-7319

*    Email: 

This message is confidential, intended only for the named 
recipient(s) and may contain information that is privileged 
or exempt from disclosure under applicable law.  If you are 
not the intended recipient(s), you are notified that the 
dissemination, distribution, or copying of this message is 
strictly prohibited.  If you receive this message in error, 
or are not the named recipient(s), please notify the sender 
at either the e-mail, fax, address, or telephone number 
listed above and delete this e-mail from your computer. 
Thank You.

Histonet mailing list

Histonet mailing list

<< Previous Message | Next Message >>