Hi,

I processed some mouse hearts that were in Z-Fix for 24 hrs. then put in
70% for anywhere from 24-48hrs. They were processed on a short run. The
epicardium  looks good but the mid myocardium is wavy.

15 min 80%

15 min 95 x 2

15 min 100% x 2

15 min Xylenes x2 

10 min paraffin x 4

Any suggestions?

Thanks.

 

 

Betsy Molinari   HT(ASCP)

Texas Heart Institute 

Cardiovascular Pathology

6770 Bertner Ave.

MC 1-283

Houston, TX 77030

832-355-6524

832-355-6812 (fax)

 

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