For those of you who use Biocare's Decloaker for HIER on regular cases (not prion protein retrieval), could you tell me how long you do HIER with these machines? I normally do my prion cases in a Decloaker for 20', with a 25' cooldown, but I suspect that regular tissues don't need that length of time at high pressure/temp. I have an extra Decloaker and am considering switching to this device for my normal case work HIER, if it's time efficient.
Please reply privately. Thanks.
Jan Shivers
Senior Scientist
Histology/IHC/EM Section Head
Pathology Teaching Program
University of Minnesota
Veterinary Diagnostic Laboratory
1333 Gortner Ave.
St. Paul, MN 55108
612-624-7297
shive003@umn.edu
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