[Histonet] (no subject)

From:"Troutman, Kenneth A"

Try the following decal from Surgipath.  We used it with great success when I was in another lab.  It is a 2 step decal procedure and it can significantly cut down on the total time.
Ashley Troutman BS, HT(ASCP)QIHC
Histopathology Laboratory
Department of Pathology
Vanderbilt University Medical Center
Nashville, TN
-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cynthia Robinson
Sent: Thursday, October 30, 2008 11:44 AM
To: histonet
Subject: [Histonet] bone marrow biopsies

We are currently using 10% formalin fixation on our bone marrow cores.  We fix for 2 hours minimum prior to decal.  We are using Immunocal from Decal Corp. for 2-4 hrs followed with processing overnight in VIP.  Cores are still crunchy upon sectioning and we are doing surface decal for up to 30 min. Our paths want cores turned out within 24 hrs following procedure. Any suggestions?


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