We are currently using 10% formalin fixation on our bone marrow cores.  We fix for 2 hours minimum prior to decal.  We are using Immunocal from Decal Corp. for 2-4 hrs followed with processing overnight in VIP.  Cores are still crunchy upon sectioning and we are doing surface decal for up to 30 min. Our paths want cores turned out within 24 hrs following procedure. Any suggestions? 
 
Thanks.
Cindi 
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