[Histonet] bone histology - preventing artifacts

From:"Alexandra Meinl"

Hello Histonetters,

When I compare my MMA bone histology with with paraffin bone sections I
notice the same artifact in the paraffin sections: The bone lining cells
seem to fall off the surface, I also noticed osteoclasts here and there that
were stripped off.
Bone morphology is good (I used EDTA for decal).
I suspect that maybe the dehydration was too fast for bone (I used the same
protocol as for normal tissue specimens).

What are your experiences with decalcified bone sections?
Do you use a different protocol for your VIP?
Or are these artifacts inevitable?


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