Hello,
Does anyone have experience with performing Immunocytochemistry (CD3, CD20) on touch preps of canine and feline lymph nodes, particularly the following:
Fixation: What reagent(s), what temperature, how long, immediately or wait until air dried, storage post fixing
Antigen Retrieval: If formalin is part of the fixative, is there a general rule for HIER on ICC specimens that relates to HIER on paraffin sections of lymph nodes for CD3 and CD20?
Thank you so very much.
Sandra Cheasty
Histology & Necrcopsy Supervisor
UW-Madison
School of Veterinary Medicine
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
|