Hi Chris-Thanks for the insight, I never would have even thought to check that the mouse isotypes were different.  I will give it a look!Kim Kim Merriam, MA, HT(ASCP)QIHCCambridge, MA ________________________________From: C.M. van der Loos To: histonet@lists.utsouthwestern.eduCc: kmerriam2003@yahoo.comSent: Tuesday, October 21, 2008 1:54:02 PMSubject: RE: anti-Human CD68Hi Kim,I wondering if your question for an anti-CD68 antibody raised in rabbit or goat is because of double staining plans. If so, please realize that the Dako antibody, clone PG-M1, is a mouse IgG3. That easily allows double staining with other mouse IgG1 or IgG2a antibodies. Go to Southern Biotechnology Associates (www. southernbiotech.com) for specific second step antibodies conjugated with alk. phos., peroxidase or biotin. Just a guess!Cheers,ChrisChris van der Loos, PhDDept. of PathologyAcademic Medical Center M2-230Meibergdreef 9NL-1105 AZ AmsterdamThe NetherlandsDate: Mon, 20 Oct 2008 11:03:44 -0700 (PDT)From: Kim Merriam Subject: [Histonet] anti-Human CD68To: Histonet histonet@lists.utsouthwestern.eduHello,I am looking for a CD68 antibody that will work in Human FFPE tissues, but it needs to be from a species other than mouse (preferably rabbit or goat). Any recommendations?Thanks,KimKim Merriam, MA, HT(ASCP)QIHCCambridge, MA       
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