We are running a congo red stain and have sodium chloride crystals precipataing onto the slide. Does anyone know why and how we can fix this? jane
"Life is short - make haste to be kind"
Dr. Jane Flinn
Director, Undergraduate Neuroscience Program
George Mason University, 3F5
4400 University Dr.
Fairfax, VA 22030
----- Original Message -----
Date: Wednesday, October 8, 2008 5:37 pm
Subject: Re: [Histonet] citrisolve?
> I have used Citrisolve for serveral years. I don't think anything
> compares to Xylene, but I do think this comes very close. I
> change it
> about once a week. It pretty much deparaffinizes in the same
> amount of
> time. Disposal is dependent on the regulations in your area.
> When I
> started using it our Environmental Health and Safety said pour it
> the drain, but as time goes by and with more regulations now I
> have to
> collect it and have it removed with hazardous waste.
> Karen Bowden
> Staff Research Associate II
> University of CA, San Diego
> Department of Orthopedics
> 9500 Gilman Dr. 0630
> La Jolla, CA 92093-0630
> 858-534-4655 voice
> 858-534-5304 fax
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> Patten, Nicole (NIH/NIAAA) [F] wrote:
> > Does anyone have any experience using Citrisolve as a Xylene
> alternative> to deparaffinize tissue sections? Do I use it just
> like Xylene for the
> > same amount of time? Can it be reused or should I use new Citrisolve
> > each time? How do I dispose of it?
> > Any help at all would be much appreciated. Thanks!
> > Nicole J. Patten
> > Post-Baccalaureate Fellow/IRTA
> > National Institutes of Health
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