[Histonet] RE: Uneven H&E Staining

From:Karla Arrington

Recently we have had an issue with uneven H&E staining.  Lately slides that have three sections on one slide, mostly GI biopsies, and the last section (bottom of slide) are paler than the upper two section.  All cut by the same person and the stains were great.  What could be the cause of this?  Also with the quality of formalin.  Can formalin be purchased as "bad" when it takes 6 days to fix placenta or breasts?  What is the cause?  Karla Arrington, HT(ASCP)      
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