Good Day Tanisha,
We use the following diastase solution and timing protocol. It is consistent and works very well.
1. Diastase Solution
Amylase (Type VI-B from Porcine Pancreas - Sigma A3176) 0.5 g (Stored in fridge at 4°C)
Tap water 50 mL
Put 50 mL of warm water from the tap (37C) into a coplin jar (check temperature of water with thermometer). Add 0.5 g of amylase and stir well. Use once and discard.
Prewarm this solution in a 37 C degree oven prior to placing slides into it for 1 hour.
Hope this will help you.
Karen J Kay, MLT
Chinook Health Laboratory
Chinook Regional Hospital
960 - 19 st South
Lethbridge, Alberta, Canada
(403) 388 - 6061 (Phone)
(403) 388 - 6067 (Fax)
> Hi All:
> I am in the process of working up a PAS Staining protocol. The PAS
> stain is working fine, but I am unable to get my tissue to digest. I
> am using .5g of Diastase in 50ml of DI water. I tried heating the
> solution in a water bath for 30 min, 1 hour and 1.5 hours and it still
> looks the same as the one without digestion. Could I be using the
> wrong Diastase? If so, does anyone have any suggestions for a new
> Tanisha Neely, HT(ASCP)
> AP-Histology/Specimen Management
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