Does anyone use agar in a procedure to prepare cell blocks from cytology material? I am interested in such a procedure as a method to help eliminate/reduce "floaters" in cell block preparations?
-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu
Sent: Monday, October 20, 2008 1:08 PM
=0ATo: histonet@lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 59, Issue 25
Send Histonet mailing list submissions to
=0A histonet@lists.utsouthwestern.edu
To subscribe or unsubscribe via the World Wide Web, visit
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
or, via email, send a message with subject or body 'help' to
histonet-request@lists.utsouthwestern.edu
You can reach the person managing the list at=0D histonet-owner@lists.utsouthwestern.edu
When replying, please edit your Subject line so it is more specific
than "Re: Contents of Histonet digest..."
Today's Topics:
=0A
1. RE: Question on Von Kossa (Patsy Ruegg)
2. Madison WI (Steven Coakley)
3. eosin pH (Hana Peter)
4. Elastin Aldehyde Fuchsin (Adam Boanas)
5. Fwd: [Histonet] Question on Von Kossa (Anne van Binsbergen)
6. Re: eosin pH (Rene J Buesa)=0D 7. Acidified Hematoxylin (Jo-Ann Bader, Ms.)
8. Re: Elastin Aldehyde Fuchsin (renafail@bellsouth.net)
9. Re: B5 fixative (Bryan Watson)
10. getting rid of biotin-avidin background (Tyrone Genade)
11. help-fibrotic tissue (godsgalnow@aol.com)
=0A 12. Re: getting rid of biotin-avidin background
(anh2006@med.cornell.edu)
13. Histology Openings in Seattle (Chris Handrahan)
14. Job Opening (Alice Bonaiuto)
15. Baker's Fluid (Jo-Ann Bader, Ms.)
16. RE: Baker's Fluid (Weems, Joyce)
17. Full Time Histotech Needed (Laurie Colbert)
18. RE: Baker's Fluid (Jo-Ann Bader, Ms.)
19. the ubiquitous yet incredibly important timer (Emily Sours)
20. RE: B5 fixative (Smith, Allen)
=0A
----------------------------------------------------------------------
Message: 1
Date: Sun, 19 Oct 2008 11:09:18 -0600=0DFrom: "Patsy Ruegg"
Subject: RE: [Histonet] Question on Von Kossa
To: "'Amos Brooks'" , ,
Message-ID: <0190942AC6114F07978BD3775EC76F49@ihctechq9h2qof>=0DContent-Type: text/plain; charset="iso-8859-1"
That is how I do VK, I put it in the window for 20 min or so. I do a
special H&E counterstain on mine, using an aqueous eosin. The eosin will=0Dlight up the osteoid by fluorescing under uv light. We use this with a
image analysis system to measure total area, calcified bone area (light
scope, from the black silver stain) osteoid seam thickness (fluorescent
scope using the eosin fluorescent property, everything else will be dark
except the osteoid seams) if you labeled the bone with a fluorchrome you can
just look at an unstained section to measure area between two labels.
Patsy
-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amos Brooks
Sent: Friday, October 17, 2008 10:15 PM
To: Herrick.James@mayo.edu; histonet@lists.utsouthwestern.edu
Subject: [Histonet] Question on Von Kossa
James,
Try this: once the sections are brought to water, 5% silver nitrate in
either bright sunlight or a 60-100 watt incandescent light bulb for 30 min
(check for browning of the control tissue adjust time as needed). Rinse well
in distilled water. 5% Sodium Thiosulfate for 1 min. Rinse and counterstain
with nuclear fast red or whatever you think would look cool :-). Dehydrate,
clear and coverslip the slides.
Of course, as with any silver stain use acid cleaned glassware and gloves
unless you like watching your fingers turn black in the sunlight. Really
well decalcified tissue usually has the Calcium washed out. Try a calcified
breast or something that wasn't decalcified. This causes microtomists to
curse a lot, but it makes great VonKossa controls.
Have fun,
Amos
Message: 10
Date: Thu, 16 Oct 2008 15:45:11 -0500
From: "Herrick, James L."
Subject: [Histonet] Question on Von Kossa
To:
Message-ID:
Content-Type: text/plain; charset="iso-8859-1"
=0A
Hello everyone,
Does anyone have a good Von Kossa stain protocol that they would not mind
sharing, on animal bone tissue (femur/tibia) that has been embedded in GMA
or MMA (sections are between 5 and 10 ?m thick)? I would appreciate it
greatly. Thank you much.=0D
Jim
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
=0D
------------------------------
Message: 2
Date: Sun, 19 Oct 2008 13:56:30 -0700 (PDT)
From: Steven Coakley
Subject: [Histonet] Madison WI
To: Histonet@lists=2Eutsouthwestern.edu
Message-ID: <160679.26224.qm@web38205.mail.mud.yahoo.com>
Content-Type: text/plain; charset=us-ascii=0D
HT(ASCP) 14 years experience looking for employment in Madison WI.
__________________________________________________
Do You Yahoo!?
Tired of spam? Yahoo! Mail has the best spam protection around
http://mail.yahoo.com
------------------------------
Message: 3
Date: Mon, 20 Oct 2008 10:29:54 +0200
From: Hana Peter
Subject: [Histonet] eosin pH
=0ATo: histonet@lists.utsouthwestern.edu
Message-ID: <48FC4182.8050507@gmail.com>
Content-Type: text/plain; charset=ISO-8859-1; format=flowed
Hi!
Can someone tell me the right pH for eosin y 1% aqueous solution?
The one I made from powder has pH 6.3. Is this OK? The commercial one we
usually buy is a bit lower (5.7).=0DThank you in advance!
Hana Peter
------------------------------
Message: 4
Date: Mon, 20 Oct 2008 11:07:56 +0100
From: "Adam Boanas"
Subject: [Histonet] Elastin Aldehyde Fuchsin
To:
Message-ID:
<22ADCE0141FB0145A580E85CE3C70BD6B5690B@ex2003.winserv.renovo-ltd.com>
Content-Type: text/plain; charset=WINDOWS-1252
Hello,
I have been using Gomori`s Aldehyde Fuchsin to stain human skin for
elastin=2E The stain works fine and is clear and specific. I am using
light green as a counterstain. My problem is that once stained, the
slides have an excess granular purple "dotted" appearance upon the
entire surface of the slide. This is not limited to the tissue position
upon the slide, but across the clear glass too. We have tried using both
=0Asuperfrost plus charged slides and uncharged slides but the problem
=0Astill is present. This precipitate is spoiling an otherwise great stain.
Does anyone have any idea about what this is and how it could be=0Dprevented? (Our next guess would be to re-filter the fuchsin solution=0Dprior to use every time)
Many thanks
=0AAdam
Adam Boanas
Histology Manager
Renovo Ltd
PLEASE NOTE OUR TELEPHONE NUMBERS ARE CHANGING -PLEASE AMEND YOUR
RECORDS:
>From Monday 07/06/2008 our Telephone number will be as follows:
Switchboard: 0161 276 7100 extension 7716
LEGAL NOTICE
This e-mail is from Renovo Group plc (Registered No 5427608 England). The information contained in this e-mail and any files transmitted with
it are confidential, may be privileged and are intended solely for the use of the individual or entity to whom they are addressed. If you are
not the intended recipient any disclosure, copying, retransmission, distribution, dissemination, action taken or omitted to be taken in reliance
upon this e-mail is strictly prohibited and may be unlawful. If you have received this communication in error please notify the sender by e-mail
or by telephone on +44(0)161 276 7100, delete it from your system and destroy any copies of it.
Please note that e-mails from Renovo Group plc pass through a virus checker but e-mails can be intercepted or affected by different viruses.
Neither Renovo Group plc nor the sender accepts any liability or responsibility for viruses or other material introduced with this message and
the recipient should ensure that the e-mail and attachments (if any) are actually virus free.
Any views expressed by an individual within this e-mail do not necessarily represent the views of Renovo Group plc. No contract may be concluded
on behalf of Renovo Group plc by means of e-mail communications.
Renovo Group plc, Manchester Incubator Building, 48 Grafton Street, Manchester, M13 9XX, UK
Tel: +44(0)161 276 7100 Fax: +44(0)161 276 7200
Website: http://www.renovo.com
------------------------------
Message: 5
Date: Mon, 20 Oct 2008 16:45:23 +0400
From: "Anne van Binsbergen"
Subject: Fwd: [Histonet] Question on Von Kossa
To: histonet
Message-ID:
Content-Type: text/plain; charset=ISO-8859-1
no - thats the really cool thing - think about it...
its that part of the bone which is usually quite hard but softens with
stewing/cooking and could be bitten off and chewed (if desired)
no demineralisation has taken place yet it is soft enough to slice with a
blade and once processed it cuts like butter on the microtome
i also have an amazing source of GMS control for fungus - an old mildewed
orange!!!
same process applies - slice off a piece, process and embed - stains on PAS
and GMS!!!
quite stunning
a pal of mine says that fish liver makes and amazing Oil red 'O' QC but have
not yet tried that one
i also hunt for liver QC material here in a country which does not do
autopsies - we trot off to the local butcher with a small jar of formalin
and pop a small slice of fresh beef liver into the formalin - am sure that
other liver would work just as wekk
some may argue that it is animal tissue but in my opinion it it not the
tissue but the glycogen we need to demo - so its not an issue - my Paths are
happy so im happy
=0D
2008/10/20 Patsy Ruegg
Do you have to decal it?
>
>
>
> Patsy Ruegg, HT(ASCP)QIHC=0D>
> IHCtech
>
> 12635 Montview Blvd. #215
>
=0A> Aurora, CO 80045
>
> 720-859-4060
>
> fax 720-859-4110
>
> pruegg@ihctech.net
>
> www.ihctech.net=0D>
> www.ihcrg.org
>
>
>
>
> ------------------------------
>
> *From:* Anne van Binsbergen [mailto:annigyg@gmail.com]
> *Sent:* Sunday, October 19, 2008 9:48 PM
> *To:* Patsy Ruegg
> *Subject:* Re: [Histonet] Question on Von Kossa=0D>
>
>
> for an excellent VK control try this:
>=0D>
>
> after you have eaten all the meat from the bones of a chicken stew - keep
> the thigh bones.
>
> Cut off the soft part on the end, pop into a casette, process as usual and
> viola!!!
>
> Try it for yourself - works like a charm
>
> ;))=0D>
> Annie
>
>
>
>
>
>
>
> 2008/10/19 Patsy Ruegg
>
> That is how I do VK, I put it in the window for 20 min or so. I do a
> special H&E counterstain on mine, using an aqueous eosin. The eosin will
> light up the osteoid by fluorescing under uv light. We use this with a
=0A> image analysis system to measure total area, calcified bone area (light
> scope, from the black silver stain) osteoid seam thickness (fluorescent
> scope using the eosin fluorescent property, everything else will be dark
> except the osteoid seams) if you labeled the bone with a fluorchrome you
> can
> just look at an unstained section to measure area between two labels.
> Patsy
>
> -----Original Message-----
> From: histonet-bounces@lists.utsouthwestern.edu
> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amos=0D> Brooks
> Sent: Friday, October 17, 2008 10:15 PM
> To: Herrick.James@mayo.edu; histonet@lists.utsouthwestern.edu
> Subject: [Histonet] Question on Von Kossa
>
> James,
> Try this: once the sections are brought to water, 5% silver nitrate in
> either bright sunlight or a 60-100 watt incandescent light bulb for 30 min
=0A> (check for browning of the control tissue adjust time as needed). Rinse
> well
> in distilled water. 5% Sodium Thiosulfate for 1 min. Rinse and counterstain
> with nuclear fast red or whatever you think would look cool :-). Dehydrate,
> clear and coverslip the slides=2E
> Of course, as with any silver stain use acid cleaned glassware and gloves
> unless you like watching your fingers turn black in the sunlight. Really
> well decalcified tissue usually has the Calcium washed out. Try a calcified
> breast or something that wasn't decalcified. This causes microtomists to
> curse a lot, but it makes great VonKossa controls.
>
> Have fun,
> Amos
>
> Message: 10
> Date: Thu, 16 Oct 2008 15:45:11 -0500
> From: "Herrick, James L."
> Subject: [Histonet] Question on Von Kossa
> To:
> Message-ID:
>
> Content-Type: text/plain; charset="iso-8859-1"
>
> Hello everyone,
>
> Does anyone have a good Von Kossa stain protocol that they would not mind
> sharing, on animal bone tissue (femur/tibia) that has been embedded in GMA
> or MMA (sections are between 5 and 10 ?m thick)? I would appreciate it
> greatly=2E Thank you much.
>
> Jim
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern=2Eedu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet=0D>
>
>
>
> --
> Anne van Binsbergen (Hope)
=0A> Abu Dhabi
> UAE
>
--
Anne van Binsbergen (Hope)
Abu Dhabi
UAE
--
Anne van Binsbergen (Hope)
Abu Dhabi
UAE
------------------------------
Message: 6
Date: Mon, 20 Oct 2008 05:59:45 -0700 (PDT)
From: Rene J Buesa
Subject: Re: [Histonet] eosin pH
To: histonet@lists.utsouthwestern.edu, Hana Peter
Message-ID: <478046.83469.qm@web65709.mail.ac4.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
The eosin should be acidic and you get to that point adding 1% aq. sol. of acetic acid to you prepared solution, at a rate of 1 mL acetic/100 mL of eosin solution.
Ren? J.
--- On Mon, 10/20/08, Hana Peter wrote:
From: Hana Peter
Subject: [Histonet] eosin pH
To: histonet@lists.utsouthwestern.edu
Date: Monday, October 20, 2008, 4:29 AM
Hi!
Can someone tell me the right pH for eosin y 1% aqueous solution?
The one I made from powder has pH 6.3. Is this OK? The commercial one we
usually buy is a bit lower (5.7).
Thank you in advance!
Hana Peter
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
__________________________________________________
=0ADo You Yahoo!?
Tired of spam? Yahoo! Mail has the best spam protection around
http://mail.yahoo.com
------------------------------
Message: 7
Date: Mon, 20 Oct 2008 09:07:24 -0400=0DFrom: "Jo-Ann Bader, Ms."
Subject: [Histonet] Acidified Hematoxylin
To:
Cc: "Melina Narlis, Ms"
Message-ID:
Content-Type: text/plain; charset="iso-8859-1"
Good Morning Everyone,
We are in dire straights We are coming to the end of out Acidified Harris Hematoxylin and I was just informed that the company that produces it will not start production until November. We will never make it. Does anyone know where I can get some?
Jo-Ann Bader
Histology Facility Coordinator=0DMcGill Cancer Center
Cancer Pavillion
1160 Pine Ave W
Rm. 3355
Montreal, QC,
Tel: 514-398-8270
Fax: 514-8398-6769
email: jo-ann.bader@mcgill.ca
=0A------------------------------
Message: 8
Date: Mon, 20 Oct 2008 14:02:13 +0000
From: renafail@bellsouth.net
Subject: Re: [Histonet] Elastin Aldehyde Fuchsin
To: "Adam Boanas" ,
Message-ID:
<102020081402.11584.48FC8F65000436C100002D4022243651029B0A02D2089B9A019C04040A0DBF04070E000E020A9D@att.net>
Content-Type: text/plain
The solution does need to be filtered before each use
Rena Fail
-------------- Original message from "Adam Boanas" : --------------
=0A
> Hello,
>
>
>
> I have been using Gomori`s Aldehyde Fuchsin to stain human skin for
> elastin. The stain works fine and is clear and specific. I am using
> light green as a counterstain. My problem is that once stained, the
> slides have an excess granular purple "dotted" appearance upon the
> entire surface of the slide. This is not limited to the tissue position
> upon the slide, but across the clear glass too. We have tried using both
> superfrost plus charged slides and uncharged slides but the problem
> still is present. This precipitate is spoiling an otherwise great stain.
=0A> Does anyone have any idea about what this is and how it could be
> prevented? (Our next guess would be to re-filter the fuchsin solution
=0A> prior to use every time)
>
>
>
> Many thanks
>
> Adam
>
>
>
> Adam Boanas
> Histology Manager
> Renovo Ltd
>
> PLEASE NOTE OUR TELEPHONE NUMBERS ARE CHANGING -PLEASE AMEND YOUR
> RECORDS:
>
> >From Monday 07/06/2008 our Telephone number will be as follows:
>
> Switchboard: 0161 276 7100 extension 7716
>
>
>
> LEGAL NOTICE
> This e-mail is from Renovo Group plc (Registered No 5427608 England). The
> information contained in this e-mail and any files transmitted with
> it are confidential, may be privileged and are intended solely for the use of
> the individual or entity to whom they are addressed. If you are
> not the intended recipient any disclosure, copying, retransmission,
> distribution, dissemination, action taken or omitted to be taken in reliance
> upon this e-mail is strictly prohibited and may be unlawful. If you have
> received this communication in error please notify the sender by e-mail
> or by telephone on +44(0)161 276 7100, delete it from your system and destroy
> any copies of it.
> Please note that e-mails from Renovo Group plc pass through a virus checker but
> e-mails can be intercepted or affected by different viruses.=0D> Neither Renovo Group plc nor the sender accepts any liability or responsibility
> for viruses or other material introduced with this message and
> the recipient should ensure that the e-mail and attachments (if any) are
> actually virus free.
> Any views expressed by an individual within this e-mail do not necessarily
> represent the views of Renovo Group plc. No contract may be concluded
> on behalf of Renovo Group plc by means of e-mail communications.
> Renovo Group plc, Manchester Incubator Building, 48 Grafton Street, Manchester,
> M13 9XX, UK
> Tel: +44(0)161 276 7100 Fax: +44(0)161 276 7200
> Website: http://www.renovo.com
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 9=0DDate: Mon, 20 Oct 2008 10:04:05 -0400
From: "Bryan Watson"
Subject: Re: [Histonet] B5 fixative
To: "Piero Nelva" ,
Message-ID: <48FC5795.ACD8.0085.0@parkview.com>
Content-Type: text/plain; charset=US-ASCII
For all of the listed B5 substitutes mentioned, how are they disposed of and are they significantly safer?
>>> "Piero Nelva" 10/17/2008 17:59 >>>
I agree. The Occ Health and SAfety representative is your best bet to
consign the B5 to history.
=0D
Piero Nelva
Anatomical pathology
Monash Medical Centre
Australia
----- Original Message -----
From: "Richard Cartun"
To: ; "Bryan Watson"
Sent: Saturday, October 18, 2008 12:46 AM
Subject: Re: [Histonet] B5 fixative
If you are having a hard time with a pathologist regarding the use of B5,
get your safety people involved. They will take care of it. We stopped
using B5 a very long time ago. We use formalin for all our lymph nodes and
bone marrows. No one should be using B5 and manufacturers should stop
making it.
Richard
Richard W. Cartun, Ph.D.
Director, Histology & Immunopathology
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT 06102
(860) 545-1596
=0A(860) 545-0174 Fax
>>> "Bryan Watson" 10/17/08 9:21 AM >>>
How many still use B5 fixative? And for those who do not, what do you use as
a replacement? One of our pathologists is insistent on using B5, and we'd
rather try to get away from it.
Thanks,
Bryan
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
=0D
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
--------------------------------------------------------------------------------
=0D
No virus found in this incoming message.
Checked by AVG - http://www.avg.com
Version: 8.0.173 / Virus Database: 270.8.1/1731 - Release Date: 10/17/2008
7:01 PM
_______________________________________________
Histonet mailing list=0DHistonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 10
Date: Mon, 20 Oct 2008 16:07:07 +0200
From: "Tyrone Genade"
Subject: [Histonet] getting rid of biotin-avidin background
To: histonet@lists.utsouthwestern.edu
Message-ID:
Content-Type: text/plain; charset=3DISO-8859-1
Hi all,
I have a problem and think I have figured out a solution to it but
need some expert advise.
=0DI'm using biotinylated GS isolectin to stain frozen brain sections for
microglia. The GS isolectin is working very well but the
avadin-fluourophore is binding to the endogenous biotin in the tissue
causing a lot of back ground. I'm thinking of fist blocking the
sections by incubating it with unlabled avidin and then following it
with another blocking step with unlabled biotin so that all the
endogenous biotin is bound to avidin and the un-endogenous biotin
bound sites of the avidin are then bound to unlabled biotin. This
would then be followed by the GS Isolectin incubation etc...
Can anyone suggest a reasonable starting concentration for the first
biotin and avidin blocking steps? Do you think this will work at all?
Kind regards=0D
--
Tyrone Genade
http://tgenade.freeshell.org
=0Aemail: tgenade@freeshell.org
tel: +27-84-632-1925 (c)
********************************************************************************=0D"For there is one God, and there is one mediator between God and
=0Amen, the man Christ Jesus, who gave himself as a ransom for all."
=0A 1 Timothy 2:5-6
------------------------------
Message: 11
Date: Mon, 20 Oct 2008 10:14:04 -0400
=0AFrom: godsgalnow@aol.com
Subject: [Histonet] help-fibrotic tissue=0DTo: histonet@lists.utsouthwestern.edu
Message-ID: <8CB00D53E12934B-784-2540@MBLK-M27.sysops.aol.com>
Content-Type: text/plain; charset="us-ascii"
I need to finally call on the experts.?
I have tried all of the tricks up my sleeve to cut extremely fibrotic Cerivical tissue.? It is not calcified, just fibrotic...it is like cutting glass.
Help.
Roxanne
=0D------------------------------
Message: 12
Date: Mon, 20 Oct 2008 14:13:18 +0000
From: anh2006@med.cornell.edu
Subject: Re: [Histonet] getting rid of biotin-avidin background
To: tgenade@freeshell.org, histonet@lists.utsouthwestern.edu
Message-ID:=0D <861309140-1224512133-cardhu_decombobulator_blackberry.rim=2Enet-1377979103-@bxe270.bisx.prod.on.blackberry>
Content-Type: text/plain
Why not use an avidin-biotin blocking kit (like those from Vector or Dako for example). They work well, are optiimized already and aren't that expensive.
-----Original Message-----
From: Tyrone Genade
Date: Mon, 20 Oct 2008 16:07:07
To:
=0ASubject: [Histonet] getting rid of biotin-avidin background
=0DHi all,
I have a problem and think I have figured out a solution to it but
need some expert advise.
I'm using biotinylated GS isolectin to stain frozen brain sections for
microglia. The GS isolectin is working very well but the
avadin-fluourophore is binding to the endogenous biotin in the tissue
causing a lot of back ground. I'm thinking of fist blocking the
sections by incubating it with unlabled avidin and then following it
with another blocking step with unlabled biotin so that all the
endogenous biotin is bound to avidin and the un-endogenous biotin
bound sites of the avidin are then bound to unlabled biotin. This
would then be followed by the GS Isolectin incubation etc...
Can anyone suggest a reasonable starting concentration for the first
biotin and avidin blocking steps? Do you think this will work at all?
Kind regards
--
Tyrone Genade
http://tgenade.freeshell.org
email: tgenade@freeshell.org
tel: +27-84-632-1925 (c)
********************************************************************************
"For there is one God, and there is one mediator between God and
men, the man Christ Jesus, who gave himself as a ransom for all."
1 Timothy 2:5-6=0D
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 13
Date: Mon, 20 Oct 2008 10:16:54 -0400
From: "Chris Handrahan"
Subject: [Histonet] Histology Openings in Seattle
To:
Message-ID:
Content-Type: text/plain; charset="us-ascii"
Looking for ASCP certified Histotechs for the following openings
day position, 7-3:30pm
2nd shift position, 6pm - 2:30am - pays $3 an hour shift differential
3rd shift position, 11pm - 7:30am - pays $10 an hour shift differential
Pathologists' Assistant Lead- 10am-6:30pm, M-F
IHC Lead - day position, at least 5+ yrs experience including 2 yrs in a
lead role.
=0D
These are all full time permanent positions. These are immediate needs
so please contact me today if you are interested!
Chris Handrahan
Managing Director of Allied Health
Healthcare Scouts
800-708-0605 office
321-231-5427 cell
chrish@healthcarescouts=2Ecom
www.healthcarescouts.com
------------------------------
Message: 14
Date: Mon, 20 Oct 2008 10:24:01 -0400
From: "Alice Bonaiuto"
=0ASubject: [Histonet] Job Opening
To:
Message-ID:
Content-Type: text/plain; charset="iso-8859-1"
=0A
Histology job opening for routine bench work &/ or IHC at Sarsota Pathology/Sarasota, Fl.
For more information go to www.sarapath=2Ecom .
Fax resumes to :(941) 362-8992 or HR@sarapath.com
=0DThanks,
Alice Bonaiuto, IHC Technical Specialist
=0A
------------------------------
=0DMessage: 15
Date: Mon, 20 Oct 2008 10:41:41 -0400
From: "Jo-Ann Bader, Ms."
Subject: [Histonet] Baker's Fluid
To:
Message-ID:
Content-Type: text/plain; charset="iso-8859-1"
Does anyone have the recepe fpr Baker's fluid?
Jo-Ann Bader
Histology Facility Coordinator
McGill Cancer Center=0DCancer Pavillion
1160 Pine Ave W
Rm. 3355
Montreal, QC,
Tel: 514-398-8270
Fax: 514-8398-6769
email: jo-ann.bader@mcgill.ca
------------------------------
Message: 16
Date: Mon, 20 Oct 2008 11:16:59 -0400
=0AFrom: "Weems, Joyce"
Subject: RE: [Histonet] Baker's Fluid
To: "Jo-Ann Bader, Ms." ,
=0A
Message-ID:
<5D64396A0D4A5346BEBC759022AAEAA50E3799@ITSSSXM01V6.one.ads.che=2Eorg>
Content-Type: text/plain; charset="us-ascii"
This was in the archives. Hope it helps!
Joyce Weems
Pathology Manager
Saint Joseph's Hospital
5665 Peachtree Dunwoody Rd NE=0DAtlanta, GA 30342
Please note new phone and fax numbers
678-843-7376 - Phone
678-843-7831 - Fax
On Wed, 24 May 2000, Barry Rittman wrote:
> what you need is "Mollifex" sold by BDH
You shouldn't use secret mixtures, but Mollifex
isn't (or isn't quite) a secret formulation. BDH
made it following a publication by J. R. Baker
(1941) A fluid for softening tissues embedded in
paraffin wax. Quart. J. Microsc. Sci=2E 61:75.
Bakers experiments led him to the composition:
Glycerol 10
60% ethyl alcohol: 90
(parts by volume).
A more recent paper is by Maria Wynnchuk (1992)
=0A in J. Histotechnol. 15(4): 321-323. She used
a mixture called Horne's modification of Baker's
solution:
95% ethanol 9520 ml
Glycerol 320 ml
Acetone 80 ml
Liquid phenol 80 ml
(I guess that the "liquid phenol" is an 80%
solution in water, not solid phenol that has
been melted by heating.) 10 litres of softening
solution seems a lot to make up! I wonder
how long it lasts for.
=0D Wynnchuk states that this mixture is better
than Baker's original and that Mollifex, which
contains a "phenol derivative" is just as good.
Anything here for Messrs Sue, Grabbit and Runne?
=0D John A. Kiernan,
Department of Anatomy & Cell Biology,
The University of Western Ontario,
LONDON, Canada N6A 5C1
=0A-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jo-Ann
Bader, Ms.
Sent: Monday, October 20, 2008 10:42 AM
To: Histonet@lists.utsouthwestern.edu
Subject: [Histonet] Baker's Fluid
Does anyone have the recepe fpr Baker's fluid?=0D
Jo-Ann Bader
Histology Facility Coordinator
McGill Cancer Center
Cancer Pavillion
1160 Pine Ave W
Rm. 3355
=0AMontreal, QC,
Tel: 514-398-8270
Fax: 514-8398-6769
email: jo-ann.bader@mcgill.ca
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Confidentiality Notice:
This email, including any attachments is the
property of Catholic Health East and is intended
for the sole use of the intended recipient(s).
It may contain information that is privileged and
confidential. Any unauthorized review, use,
disclosure, or distribution is prohibited. If you are
not the intended recipient, please reply to the
sender that you have received the message in
error, then delete this message.
=0A
------------------------------
Message: 17
Date: Mon, 20 Oct 2008 08:18:45 -0700
From: "Laurie Colbert"
Subject: [Histonet] Full Time Histotech Needed
To:
Message-ID:
<57BE698966D5C54EAE8612E8941D768303F54E97@EXCHANGE3.huntingtonhospital.com>
Content-Type: text/plain; charset="us-ascii"
Huntington Hospital in Pasadena, CA has a full time histotech opening
for an experienced histotech (2+ years). The position is Monday-Friday,
5:30 am - 2:00 pm, with on-call every 5th Saturday=2E Experienced
applicants may contact me at the number below.
=0A
Laurie Colbert
Huntington Hospital
=0APasadena, CA 91105
(626) 397-8620
------------------------------
Message: 18
Date: Mon, 20 Oct 2008 11:34:56 -0400
From: "Jo-Ann Bader, Ms."
Subject: RE: [Histonet] Baker's Fluid
To: "Weems, Joyce"
Cc: Histonet@lists.utsouthwestern.edu
Message-ID:
Content-Type: text/plain; charset="iso-8859-1"
Thanks that is the one I am looking for!
Jo-Ann Bader
Histology Facility Coordinator
McGill Cancer Center
=0ACancer Pavillion
1160 Pine Ave W
Rm. 3355
Montreal, QC,=0DTel: 514-398-8270
Fax: 514-8398-6769
email: jo-ann.bader@mcgill.ca
-----Original Message-----
From: Weems, Joyce [mailto:JWeems@sjha.org]
Sent: Mon 10/20/2008 11:16 AM
To: Jo-Ann Bader, Ms.; Histonet@lists.utsouthwestern.edu
=0ASubject: RE: [Histonet] Baker's Fluid
This was in the archives. Hope it helps!
Joyce Weems
Pathology Manager
Saint Joseph's Hospital
5665 Peachtree Dunwoody Rd NE
Atlanta, GA 30342
Please note new phone and fax numbers
678-843-7376 - Phone
=0A678-843-7831 - Fax
On Wed, 24 May 2000, Barry Rittman wrote:
> what you need is "Mollifex" sold by BDH
=0A
You shouldn't use secret mixtures, but Mollifex
isn't (or isn't quite) a secret formulation. BDH
made it following a publication by J. R. Baker
(1941) A fluid for softening tissues embedded in
paraffin wax. Quart. J. Microsc. Sci. 61:75.
Bakers experiments led him to the composition:
Glycerol 10
60% ethyl alcohol: 90
(parts by volume).=0D A more recent paper is by Maria Wynnchuk (1992)
in J. Histotechnol. 15(4): 321-323. She used
a mixture called Horne's modification of Baker's
solution:
95% ethanol 9520 ml
=0A Glycerol 320 ml
Acetone 80 ml
Liquid phenol 80 ml
(I guess that the "liquid phenol" is an 80%
solution in water, not solid phenol that has
been melted by heating.) 10 litres of softening
solution seems a lot to make up! I wonder
how long it lasts for.
Wynnchuk states that this mixture is better
than Baker's original and that Mollifex, which
contains a "phenol derivative" is just as good.
Anything here for Messrs Sue, Grabbit and Runne?
John A. Kiernan,
Department of Anatomy & Cell Biology,
The University of Western Ontario,
LONDON, Canada N6A 5C1
-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jo-Ann
=0ABader, Ms.
Sent: Monday, October 20, 2008 10:42 AM
To: Histonet@lists.utsouthwestern.edu
Subject: [Histonet] Baker's Fluid
=0A
Does anyone have the recepe fpr Baker's fluid?
Jo-Ann Bader
Histology Facility Coordinator
McGill Cancer Center
Cancer Pavillion
1160 Pine Ave W
Rm. 3355
Montreal, QC,
Tel: 514-398-8270
Fax: 514-8398-6769
email: jo-ann.bader@mcgill=2Eca
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
=0Ahttp://lists.utsouthwestern.edu/mailman/listinfo/histonet
Confidentiality Notice:
This email, including any attachments is the
property of Catholic Health East and is intended
for the sole use of the intended recipient(s).
It may contain information that is privileged and
confidential. Any unauthorized review, use,
disclosure, or distribution is prohibited. If you are
not the intended recipient, please reply to the
sender that you have received the message in
=0Aerror, then delete this message.
=0D------------------------------
Message: 19
Date: Mon, 20 Oct 2008 11:59:06 -0400
From: "Emily Sours"
Subject: [Histonet] the ubiquitous yet incredibly important timer=0DTo: histonet@lists.utsouthwestern.edu
Message-ID:
Content-Type: text/plain; charset=UTF-8
We're in the market for new timers. We have been using this
kindfrom
Fisher, which is exactly what we need but they break very easily.
What we like about these is
-when the time runs out and the alarm beeps for one minute the sound stops
but the timer continues to count up until you stop it
-you DON'T enter the time by pushing the buttons a certain number of times=0D(ie, push minute button 30 times for 30 minutes)
-they run on one AAA battery
-the time display is pretty big
-they have a magnet on the back
Emily
--
i'll render services that you may reasonably require
http://www.youtube.com/watch?v=t_at7lEGpjg=0D
------------------------------
Message: 20
=0ADate: Mon, 20 Oct 2008 12:02:51 -0400
From: "Smith, Allen"
Subject: RE: [Histonet] B5 fixative
To: 'Bryan Watson'
Cc: "'histonet@lists.utsouthwestern.edu'"
=0AMessage-ID:
Content-Type: text/plain; charset="us-ascii"
B5, Schaudinn's, SUSA, Helly's, and Zenker's fixatives contain mercury (II) ions, which are VERY poisonous. Sewage bacteria convert mercury (II) to methyl mercury, which is very, very poisonous=2E (For a shocking example of mercury toxicity, see the photo essay MINAMATA by W. Eugene Smith.) When these fixatives are used, the fixative and the first washing must be given to a waste disposal company that can recycle it.
B5 substitutes substitute a less poisonous ion, usually zinc (II), for mercury. These substitutes contain other things that are more poisonous than zinc. I store them as if they were purely the most poisonous component and give them to a waste disposal company twice a year.
=0A
-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bryan Watson
Sent: Monday, October 20, 2008 10:04 AM
To: Piero Nelva; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] B5 fixative
For all of the listed B5 substitutes mentioned, how are they disposed of and are they significantly safer?
>>> "Piero Nelva" 10/17/2008 17:59 >>>
I agree. The Occ Health and SAfety representative is your best bet to
consign the B5 to history.
Piero Nelva
Anatomical pathology
Monash Medical Centre
Australia
----- Original Message -----
From: "Richard Cartun" =0DTo: ; "Bryan Watson"
Sent: Saturday, October 18, 2008 12:46 AM=0DSubject: Re: [Histonet] B5 fixative
If you are having a hard time with a pathologist regarding the use of B5,
get your safety people involved. They will take care of it. We stopped
using B5 a very long time ago. We use formalin for all our lymph nodes and
=0Abone marrows. No one should be using B5 and manufacturers should stop=0Dmaking it.
Richard
Richard W. Cartun, Ph.D.
Director, Histology & Immunopathology
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT 06102
(860) 545-1596
(860) 545-0174 Fax
>>> "Bryan Watson" 10/17/08 9:21 AM >>>
How many still use B5 fixative? And for those who do not, what do you use as
=0Aa replacement? One of our pathologists is insistent on using B5, and we'd
rather try to get away from it.
Thanks,
Bryan
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------------------------------
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End of Histonet Digest, Vol 59, Issue 25
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