Dear Histonetters,
You may recall my earlier post on rings of pig aorta. I have managed to
keep them alive quite nicely with DMEM many thanks for the tips.
Unfortunately, I am still struggling to stain for endothelial adhesion
molecules.
I have read and received various different opinions on fixation after I have
made my cryostat sections. Should the slides be fixed immediately, or air
dried first? Acetone or formalin?
Any tips would be gratefully received.
Thank You,
Alistair
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