[Histonet] Pig endothelium....again

From:Alistair Lindsay



Dear Histonetters,

You may recall my earlier post on rings of pig aorta.  I have managed to
keep them alive quite nicely with DMEM  many thanks for the tips.

Unfortunately, I am still struggling to stain for endothelial adhesion
molecules.

I have read and received various different opinions on fixation after I have
made my cryostat sections.  Should the slides be fixed immediately, or air
dried first? Acetone or formalin?

Any tips would be gratefully received.

Thank You,
Alistair



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