Hi All:
 
I am in the process of working up a PAS Staining protocol. The PAS stain
is working fine, but I am unable to get my tissue to digest. I am using
.5g of Diastase in 50ml of DI water. I tried heating the solution in a
water bath for 30 min, 1 hour and 1.5 hours and it still looks the same
as the one without digestion. Could I be using the wrong Diastase? If
so, does anyone have any suggestions for a new product?
 
Thanks,
 
Tanisha Neely, HT(ASCP)
AP-Histology/Specimen Management
   




-----------------------------------------------------
Confidentiality Notice: This e-mail transmission 
may contain confidential or legally privileged 
information that is intended only for the individual 
or entity named in the e-mail address. If you are not 
the intended recipient, you are hereby notified that 
any disclosure, copying, distribution, or reliance 
upon the contents of this e-mail is strictly prohibited. 

If you have received this e-mail transmission in error, 
please reply to the sender, so that we can arrange 
for proper delivery, and then please delete the message 
from your inbox. Thank you.