I have a question for anyone out in
histoland that manually processes tissue. I have a study that will be
using radioactive I125 mouse brain tissue and I don't want to process
these in our Lecia TP 1050 auto processor for risk of contaminating the
unit. The other researcher in the lab would not like me much if
contaminated our only processor......
So I need a good quick manual process protocol for brain, spinal cord,
liver tissues. I have been using paraformaldehyde fixed frozen brains and
sectioning on the cryostat but I want the best morphology and best
sections I can get so I'm thinking of trying paraffin. Should I use a
desiccation/vacuum chamber to increase my infiltration? Since I'm using
I125 with a 1/2 life of 59 days I want to get these processed quickly so
I can do microautoradiography...
Any help is greatly appreciated...
Sr. Research Associate II
Abbott Bioresearch Center
100 Research Drive
Worcester, MA 01605-4341
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