[Histonet] Human anti Human

From:"Amos Brooks"

Hi,   I should start by saying: I have not done this, but this is the way Iwould approach this problem if I were to encounter it.   First I assume you have an human antibody raised in humans right? So, youneed an antibody that will detect the human antibody like "Anti-Human IgG(-chain specific) antibody produced in rabbit" or "Monoclonal Anti-HumanIgG (-chain specific) antibody produced in mouse". (As seen here:http://www.sigmaaldrich.com/life-science/cell-biology/antibodies/antibody-products.htm?TablePage=14574992.Then you can detect the Rabbit or Mouse secondary with your usual antiRabbit or Mouse detection. (I would use a polymer just to minimize biotinbackground.)   If anyone sees any holes in that line of approach, I'd love to hear otherideas. It's an interesting problem.Best of luck,Amos Brooks Message: 22Date: Fri, 24 Oct 2008 09:57:24 -0700From: "BJON (Brian Johnson)" Subject: [Histonet] Human anti HumanTo: histonet@lists.utsouthwestern.eduMessage-ID: <746E68D5CBB205409B12EC32A61EE4010A2FE8E7@ned.zgi.com>Content-Type: text/plain; charset=us-asciiHelloI am wondering if anyone has found, and or used, an IHC polymer kit fora human anti human monoclonal antibody.  Beyond the polymer kit, doesanyone have any tips for using a human anti human antibody for IHCwithout biotinylating it?Thank you.Brian Johnson

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