A student in my lab is doing some work in another lab here and having 
some problems with coverslipping their H&E sections. They are using 
HistoClear 1X as a clearing agent and Cytoseal mounting medium. From 
looking at the coverslipped slides I'm guessing that these two 
products are not compatible.
The mounting medium is sort of clumping up under coverslips and has a 
hazy appearance. Yes, I did also find a bit of water in their 
HistoClear as well.
Does anybody else have any experience with HistoClear 1X and/or does 
anybody know what mounting medium is good to use with the product? I 
suggested that they call the company who manufactures HistoClear but 
I don't know if they have done that yet.

Thanks,

Andi Grantham
.....................................................................
: Andrea Grantham, HT(ASCP)     Dept. of Cell Biology & Anatomy     :
: Sr. Research Specialist       University of Arizona               :
: (office:  AHSC 4212)          P.O. Box 245044                     :
: (voice:  520-626-4415)        Tucson, AZ  85724-5044    USA       :
: (FAX:  520-626-2097)          (email:  algranth@u.arizona.edu)       :
:...................................................................:
           http://www.cba.arizona.edu/histology-lab.html


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