I have a researcher in my lab that transfected cells with EGFP, injected them into mouse and then 3 weeks later harvested the tissue and frozen it in OCT as usual. After sectioning, he air dried the slides for 1 hour and then dried them at 60 degrees C for 1 hour. He was hoping just to see his EGFP infected cells which are bright green, however, the autofluorescence is everywhere - both with GFP and Rhodamine filters. Our question is - did the heating in the oven cause increased autofluorescence?
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