We have been using the microwave HIER and have had good results, however when our microwave bits the dust I would like to have a pressure cooker method in place. I feel the pressure cooker is more consistent for all the slides. We are a veterinary diagnostic lab and I would like to have some idea of where to begin. I have looked at different protocols and they often indicate HIER in a pressure cooker but do not give the details. I currently use citrate buffer pH 6. I put the slides in refrigerated buffer and microwave on high for 1 min 45 sec. or until the buffer just starts to boil. I then set the microwave on 10% power for 10 minutes. Afterward the slides are allowed to cool in the microwave for 1 hour. We have a biocare Decloaker Chamber and I would appreciate help with the program I should use. Do you start with cold buffer or should I prewarm it? What temperature should I use? How long should I maintain the temperature? How long should it be before I remove the slides?
I also am working with a new protocol that calls for heating in a steamer. Should the temperature of the buffer be warm, cold or room temp when I start?
Margaret Perry HT (ASCP)
IHC Lab Manager Veterinary Science
Animal Disease Research and Diagnostic Lab
South Dakota State University
Box 2175 North Campus Drive
Brookings SD 57007
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