Addendum... I should have included this information in my original posting...
I'm already doing a 3% H2O2 peroxidase quench (10-15 minutes) and a 10% normal serum protein block prior to primary Ab, and a 2-4% case species serum addition to the link Ab.
Is there something ELSE that I could use to block binding to platelets (or what appears to be platelets, though it could be bits of proteinaceous cellular debris that's binding Ab nonspecifically)?
----- Original Message -----
From: Jan Shivers
Sent: Tuesday, October 07, 2008 3:14 PM
Subject: platelets and IHC
Has anyone ever encountered platelets staining nonspecifically when using an antibody directed against other antigens? If so, how do you quench that activity? Thanks.
Histology/IHC/EM Section Head
University of Minnesota
Veterinary Diagnostic Laboratory
1333 Gortner Ave.
St. Paul, MN 55108
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