Addendum... I should have included this information in my original posting...

I'm already doing a 3% H2O2 peroxidase quench (10-15 minutes) and a 10% normal serum protein block prior to primary Ab, and a 2-4% case species serum addition to the link Ab.  

Is there something ELSE that I could use to block binding to platelets (or what appears to be platelets, though it could be bits of proteinaceous cellular debris that's binding Ab nonspecifically)?  

Jan S

----- Original Message ----- 
From: Jan Shivers 
To: histonet 
Sent: Tuesday, October 07, 2008 3:14 PM
Subject: platelets and IHC


Hi all,

Has anyone ever encountered platelets staining nonspecifically when using an antibody directed against other antigens?  If so, how do you quench that activity?  Thanks.

Jan Shivers
Senior Scientist
Histology/IHC/EM Section Head
University of Minnesota
Veterinary Diagnostic Laboratory
1333 Gortner Ave.
St. Paul, MN  55108
612-624-7297
shive003@umn.edu
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