Thanks for all your good advice on CD4. I've finally got a stain I'm happy
to look at. For the record, the technique is 4B12 clone, Standard retrieval
and 42 minutes incubation, followed by an amplification step. Another big
improvment was using a fresh (ie cut at the moment) control, instead of a
stock (ie cut about two weeks before).
Any thoughts on the use of precut controls in general?
Monash Medical Centre
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