Dear Histonetters

Thanks for all your good advice on CD4.  I've finally got a stain I'm happy 
to look at.  For the record, the technique is 4B12 clone, Standard retrieval 
and 42 minutes incubation, followed by an amplification step.  Another big 
improvment was using a fresh (ie cut at the moment) control, instead of a 
stock (ie cut about two weeks before).

Any thoughts on the use of precut controls in general?

Regards

Piero Nelva
Anatomical Pathology
Monash Medical Centre
Australia 


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