Fwd: [Histonet] Question on Von Kossa

From:"Anne van Binsbergen"



 no - thats the really cool thing - think about it...
its that part of the bone which is usually quite hard but softens with
stewing/cooking and could be bitten off and chewed (if desired)
no demineralisation has taken place yet it is soft enough to slice with a
blade and once processed it cuts like butter on the microtome

i also have an amazing source of GMS control for fungus - an old mildewed
orange!!!
same process applies - slice off a piece, process and embed - stains on PAS
and GMS!!!
quite stunning

a pal of mine says that fish liver makes and amazing Oil red 'O' QC but have
not yet tried that one

i also hunt for liver QC material here in a country which does not do
autopsies - we trot off to the local butcher with a small jar of formalin
and pop a small slice of fresh beef liver into the formalin - am sure that
other liver would work just as wekk

some may argue that it is animal tissue but in my opinion it it not the
tissue but the glycogen we need to demo - so its not an issue - my Paths are
happy so im happy



2008/10/20 Patsy Ruegg 

  Do you have to decal it?
>
>
>
> Patsy Ruegg, HT(ASCP)QIHC
>
> IHCtech
>
> 12635 Montview Blvd. #215
>
> Aurora, CO 80045
>
> 720-859-4060
>
> fax 720-859-4110
>
> pruegg@ihctech.net
>
> www.ihctech.net
>
> www.ihcrg.org
>
>
>
>
>  ------------------------------
>
> *From:* Anne van Binsbergen [mailto:annigyg@gmail.com]
> *Sent:* Sunday, October 19, 2008 9:48 PM
> *To:* Patsy Ruegg
> *Subject:* Re: [Histonet] Question on Von Kossa
>
>
>
> for an excellent VK control try this:
>
>
>
> after you have eaten all the meat from the bones of a chicken stew - keep
> the thigh bones.
>
> Cut off the soft part on the end, pop into a casette, process as usual and
> viola!!!
>
> Try it for yourself - works like a charm
>
> ;))
>
> Annie
>
>
>
>
>
>
>
> 2008/10/19 Patsy Ruegg 
>
> That is how I do VK, I put it in the window for 20 min or so.  I do a
> special H&E counterstain on mine, using an aqueous eosin.  The eosin will
> light up the osteoid by fluorescing under uv light.  We use this with a
> image analysis system to measure total area, calcified bone area (light
> scope, from the black silver stain) osteoid seam thickness (fluorescent
> scope using the eosin fluorescent property, everything else will be dark
> except the osteoid seams) if you labeled the bone with a fluorchrome you
> can
> just look at an unstained section to measure area between two labels.
> Patsy
>
> -----Original Message-----
> From: histonet-bounces@lists.utsouthwestern.edu
> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amos
> Brooks
> Sent: Friday, October 17, 2008 10:15 PM
> To: Herrick.James@mayo.edu; histonet@lists.utsouthwestern.edu
> Subject: [Histonet] Question on Von Kossa
>
> James,
>     Try this: once the sections are brought to water, 5% silver nitrate in
> either bright sunlight or a 60-100 watt incandescent light bulb for 30 min
> (check for browning of the control tissue adjust time as needed). Rinse
> well
> in distilled water. 5% Sodium Thiosulfate for 1 min. Rinse and counterstain
> with nuclear fast red or whatever you think would look cool :-). Dehydrate,
> clear and coverslip the slides.
>   Of course, as with any silver stain use acid cleaned glassware and gloves
> unless you like watching your fingers turn black in the sunlight. Really
> well decalcified tissue usually has the Calcium washed out. Try a calcified
> breast or something that wasn't decalcified. This causes microtomists to
> curse a lot, but it makes great VonKossa controls.
>
> Have fun,
> Amos
>
> Message: 10
> Date: Thu, 16 Oct 2008 15:45:11 -0500
> From: "Herrick, James L." 
> Subject: [Histonet] Question on Von Kossa
> To: 
> Message-ID:
>       
> Content-Type: text/plain;       charset="iso-8859-1"
>
> Hello everyone,
>
> Does anyone have a good Von Kossa stain protocol that they would not mind
> sharing, on animal bone tissue (femur/tibia) that has been embedded in GMA
> or MMA (sections are between 5 and 10 Ám thick)? I would appreciate it
> greatly. Thank you much.
>
> Jim
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>
>
> --
> Anne van Binsbergen (Hope)
> Abu Dhabi
> UAE
>



-- 
Anne van Binsbergen (Hope)
Abu Dhabi
UAE



-- 
Anne van Binsbergen (Hope)
Abu Dhabi
UAE
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