FW: [Histonet] Can you believe it

From:"Woodward, Denise"

-----Original Message-----
From: Woodward, Denise 
Sent: Wednesday, November 26, 2008 2:00 PM
To: 'Webb, Dorothy L'
Subject: RE: [Histonet] Can you believe it

Yes I Have heard of total disintegration. 
A histologist friend in Maine told me about having to take the heat knob
off a combination heat/stir plate when the heat knob was turned on
instead of the stir knob and the cassettes of bone to be decalcified
completely disintegrated by morning.  This happened twice. The heat knob
was removed before the third occurrence could transpire!
Denise Long Woodward

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Webb,
Dorothy L
Sent: Tuesday, November 25, 2008 4:03 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Can you believe it

Has anyone ever heard of cassettes and tissue totally disintegrating in
formic acid decal solution with a high heat on the solution for about 3
hours?????  We had 3 cassettes with tissue for decal placed in our
container on the platform for adding agitation.  Someone had moved the
knob to heat and this wasn't noticed until about 2-3 hours later, when
it was boiling!  We cannot locate the cassettes (I think someone
misplaced them) and others think they totally disintegrated with the
decal and heat...what does everyone think?????  In all of my years of
histology, never have I heard of anything so weird ......

Dorothy Webb
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