i agree - with gravity you have little chance of perfusing at too  
high a pressure; for neurotox/neuropath it makes the brains  
particularly fix much better.

Cheryl

Cheryl Cross, DVM, Dipl. ACVP
Researcher
University Corporation for Atmospheric Research
College of Veterinary Medicine
University of Tennessee Department of Pathology
2407 River Drive, Room A201
Knoxville, TN 37996-4542
(423) 967-2724
fax: 865-974-5616
ccross@ucar.edu





On Oct 31, 2007, at 9:42 AM, Kemlo Rogerson wrote:

> Why not use the force of gravity; if you put a header tank 1 metre  
> about
> the animal and introduce the fixative from that isn't that one
> atmosphere of pressure? Thought it was as that's how I perfused
> collier's lungs when we doing pneumoconiosis studies for compensation.
>
> Kemlo Rogerson
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