Re: [Histonet] immunohistochemistry method

From:Rene J Buesa



Check your titration "checker-board" against a good membrane positive control, and use the highest dilution that stains just the membrane, and use that dilution in another test with the same tissue you are having the problem.
  Probably you are using too much antibody (more than required)..
  Ren J.

docqian  wrote:
  Dear all,

I have one question to ask for your help.
I would like to check one antibody with immunohistochemistry method in paraffin-embedded tissues and this antibody is a membrane protein, however after this staing, I find that there are also strong stainings in cytoplasm, I do not know the reason. Does anyone have some ideas about it? And how can I only label the membrane?

Thank you!

Guofeng


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