Yes, if there is a space beyween the OCT and the tissue, you can "refill" it and freeze it again.
  René J.

Atoska Gentry  wrote:
  hello, has anyone observed empty space surrounding tissue frozen in 
O.C.T. or similar tissue freezing medium after snap freezing? Upon 
sectioning samples shipped previously snap frozen to one of our 
researchers I noticed space surrounding various areas of the tissue. 
Does anyone know what probably caused this? Could this be a factor in 
the generation of horrible sections? I suspect the tissue may be moving 
when the blade touches it. Is there a know remedy that will not damage 
the tissue? Has anyone ever added O.C.T. to already frozen tissues? Any 
assistance will be much appreciated. Atoska

-- 
Atoska S. Gentry, B.S., HT(ASCP)
Research Assistant IV
Scott-Ritchey RSCH Center
College of Vet. Med
Auburn, AL 36849
PH (334) 844-5579
FAX (334) 844-5850
email: gentras@vetmed.auburn.edu


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