Re: [Histonet] frozen sections

From:Rene J Buesa

Yes, if there is a space beyween the OCT and the tissue, you can "refill" it and freeze it again.
  René J.

Atoska Gentry  wrote:
  hello, has anyone observed empty space surrounding tissue frozen in 
O.C.T. or similar tissue freezing medium after snap freezing? Upon 
sectioning samples shipped previously snap frozen to one of our 
researchers I noticed space surrounding various areas of the tissue. 
Does anyone know what probably caused this? Could this be a factor in 
the generation of horrible sections? I suspect the tissue may be moving 
when the blade touches it. Is there a know remedy that will not damage 
the tissue? Has anyone ever added O.C.T. to already frozen tissues? Any 
assistance will be much appreciated. Atoska

Atoska S. Gentry, B.S., HT(ASCP)
Research Assistant IV
Scott-Ritchey RSCH Center
College of Vet. Med
Auburn, AL 36849
PH (334) 844-5579
FAX (334) 844-5850

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