Re: [Histonet] curious about soaking paraffin blocks.

From:"Anna K. Schultz"

Thanks a lot for the responses and I am definitely enjoying the 'debate.'

Kaliko, Bonnie wrote:
> Well as far as animal tissue is concerned, ice and water are needed to
> hydrate the tissue in order to obtain a section.  Each tissue responds
> differently, some needing time on the ice longer then others.  
> -----Original Message-----
> From:
> [] On Behalf Of Kemlo
> Rogerson
> Sent: Thursday, October 25, 2007 10:41 AM
> To: Mike Pence; Cheri Miller; Smith Wanda; Anna K. Schultz;
> Subject: RE: [Histonet] curious about soaking paraffin blocks.
> "So if you place the block on the ice tissue up, what are you soaking,
> paraffin?"
> Well if soak it in ice tissue side down what are you cutting frozen
> sections? Don't understand why you'd want to potentially stick water
> back into something you've dehydrated; makes no sense. Why not just put
> blocks face down on a chiller or one of those Peltier effect thingies. 
> Kemlo Rogerson
> Pathology Manager
> DD   01934 647057 or extension 3311
> Mob 07749 754194; Pager 07659 597107;
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Anna K. Schultz
SDRC Core A Research Technician
Department of Dermatology
College of Physicians and Surgeons
Columbia University
Vanderbilt Clinic 15-211
630 West 168^th Street
New York, NY 10032
Phone: (212) 305-4954

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