i see - thanks Bonnie and Rene for your input...
The old trial sections have not only a lower dilution that didn't
work, but a completely different antibody. the rest of the protocol
would be the same.
it sounds as though if my old sections were understained it may be
worth a shot.....but no guarantees :) if i'm understanding you
Cheryl Cross, DVM, Dipl. ACVP
University Corporation for Atmospheric Research
College of Veterinary Medicine
University of Tennessee Department of Pathology
2407 River Drive, Room A201
Knoxville, TN 37996-4542
On Oct 16, 2007, at 4:58 PM, Rene J Buesa wrote:
> If the section already has DAB (as it probably does), you cannot
> eliminate it.
> René J.
> Cheryl Cross wrote:
> hi everybody -
> I'm going back through some of my earlier attempts at immuno for
> cleaved caspase 3; i have a beautiful lesion i ruined :) on
> dilutions. is there anyway i can use my newer, better protocol on
> this previously stained slide?
> seems such a waste :)
> thanks for any input!
> Cheryl Cross, DVM, Dipl. ACVP
> University Corporation for Atmospheric Research
> College of Veterinary Medicine
> University of Tennessee Department of Pathology
> 2407 River Drive, Room A201
> Knoxville, TN 37996-4542
> (423) 967-2724
> fax: 865-974-5616
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