Re: [Histonet] bleeding eosin

From:Jennifer MacDonald

Not all mounting medium is compatible with xylene substitutes.  Certain 
combinations of mounting media and substitute can cause the bleeding of 

Jennifer MacDonald
Director, Histotechnician Training Program
Mt. San Antonio College
1100 N. Grand Ave.
Walnut, CA 91789
(909) 594-5611 ext. 4884

Rene J Buesa  
Sent by:
10/29/2007 09:24 AM


Re: [Histonet] bleeding eosin

That is a known disadvantage of some xylene substitutes.
  René J. wrote:
  Hi - 
I was wondering if anyone who uses xylene substitutes have had problems 
with the eosin bleeding out of the tissue. I don't know if it is the type 
and brand of eosin or something else. I have never used a substitute 
before and I find that the stainer needs to be changed much more that with 
regular xylene. I seem to get a lot of water on the slides unless I change 
it every other day.
Paramus, NJ
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