I would check the antibody dilution, it is probably too low.
  René J.

Diana McCaig  wrote:
  Hi
We are using the Biocare IHC protocol and using Fisher superfrost plus
slides (due to decloaking). When doing the double stain, there is a lot
of background stain from the DAB. We do wash well so that is not the
problem. I am wondering if is the charge on the slides. I have checked
the pH of all the solutions and they are okay. Any ideas. The stain is
readable, but does not look too nice.
thanks
Diana
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