Re: [Histonet] Tris buffer

From:Rene J Buesa

The idea is that the slides you have hydrated (after dewaxing) should be immersed (soaked if you like) in a liquid with a neutral pH (either Tris or PBS).
  René J.

sheila adey  wrote:
Hello netters.

Can anyone tell me the function of the pre soak in Tris buffer, prior to the IHC? Also, how long are you pre soaking your IHCs?

Thanks in advance

Sheila Adey HT MLTPort Huron HospitalMichigan
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