It is not trivial, but is not impossible. A quick
Google search of the terms "staining epoxy sections"
turned up over 600K responses. A search of PubMed
might be more to the point. The first paper I saw
referenced use of H2O2 to facilitate H&E staining.
Have not tried it myself. References I was more
familiar with used either sodium methoxide or ethoxide
to remove the plastic. Staining was pretty much
straightforward after that. These techniques involve
hazardous chemicals. A more useful method would be to
use one of the polychromatic stains also found in the
Google search I mentioned at the outset. Sorry, but I
can't provide specific references--those are long gone
along with the job.
Roger Moretz, Ph.D.
Electron Microscopist (ret.)
"I'm not dead yet" Monty Python, Search for the Holy
--- Lawrence Mason wrote:
> I have some archived mouse tissues in Embed 812
> resin. Is it possible to stain these tissues with
> hematoxylin and eosin? I was unable to find any
> relevant info in the archives.
> Larry Mason
> Wyeth Research
> Cambridge, MA
> Histonet mailing list
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