PERHAPS your problem resides in the antibody titer you are using and which tissue to used to determine it. Variations on the epitope concentration and changes in the target tissue can cause this problem.
  René J.
  

sheila adey  wrote:
  
Hi Everyone,

We've been having on going issues with our Negative tissues staining positive. Today the control that had no retrieval was perfect. The slide that was pepsin retrieved was quite positive and the one that was HIER was a little positive. 

Just a note here, this is the i6000 from Biogenex and the tech support is very inefficient. Very nice people but .....

Any ideas?

Thanks in advanceSheila Adey HT MLTPort Huron HospitalMichigan
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