Igor,These processing times might be too long, I would try closer to 30-35 minutes per station for mouse skins. Kim Merriam, MA, HT(ASCP)=0ACambridge, MA----- Original Message ----From: Igor Deyneko To: Histonet@lists.utsouthwestern.eduSent: Friday, October 19, 2007 1:34:34 PMSubject: [Histonet] Mouse Skin ProcessingDear Histonetters!I have been havins some problems with processing mouse skin. The blacks aredifficult to section, then tissue starts flowing away from the paraffin andthe sections do not retain Hematoxylin stain. That suggest to me that theprocessing and paraffin infiltration were poor.Here's the processing protocol I used for pieces no larger than 1cm^2.70 % Alcohol - 1.5 hr70 % Alcohol - 1.5 hr70 % Alcohol - 1.0 hr80 % Alcohol - 1.0 hr95 % Alcohol - 1.0 hr95 % Alcohol - 1.0 hr100 % Alcohol - 1.5 hr100 % Alcohol - 1.5 hr( Due to machine only having 2 stations for thatalcohol)Xylene - 1.5 hrXylene - 1.5 hr(Due to machine having only 2 stations for Xylene)Paraffin - 1.0 hrParaffin - 1.0 hrParaffin - 1.0 hrAny suggestions would be greately appreciated.Thank you.Igor DeynekoInfinity PharmaceuticalsCambridge, MA._______________________________________________Histonet mailing listHistonet@lists.utsouthwestern.eduhttp://lists.utsouthwestern.edu/mailman/listinfo/histonet__________________________________________________Do You Yahoo!?Tired of spam?  Yahoo! Mail has the best spam protection around http://mail.yahoo.com 
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet