Re: [Histonet] I NEED CDX2 CONTROL IHC

From:"Richard Cartun"



We use a section of colon with colonic adenocarcinoma that also contains normal epithelium.  If you are seeing weak staining you probably are not achieving optimal antigen retrieval.  We use BioGenex's mAb at 1:200 following high pH HIER on the Bond Max and obtain excellent immunoreactivity.

Richard

Richard W. Cartun, Ph.D.
Director, Immunopathology & Histology
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 545-1596
(860) 545-0174 Fax

>>> Babatunde Ajibade  10/18/07 5:44 AM >>>
Can antibody  tell me the best CDX2  control  tissue, I have been try to get control for this  antigen, I have tried  colon  from diferent samples but I have not be able to get good control, its stain faintly.
  Thank you in advance.
   
  Tunde Ajibade.
   

 __________________________________________________
Do You Yahoo!?
Tired of spam?  Yahoo! Mail has the best spam protection around 
http://mail.yahoo.com 
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu 
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Confidentiality Notice

This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or proprietary information which is legally privileged.  Any unauthorized review, use, disclosure, or distribution is prohibited.  If you are not the intended recipient, please promptly contact the sender by reply e-mail and destroy all copies of the original message.

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet




<< Previous Message | Next Message >>