Re: [Histonet] Help regarding staining of BBB

From:John Kiernan

The term blood-brain barrier refers to the failure of substances to pass from the blood into the nervous tissue of the CNS. Such substances include most large molecules such as plasma proteins, and dyes that bind to plasma proteins. The older dyes used for the purpose, such as Evans blue and trypan blue, can be lost in the preparation of sections. From about the 1960s, the popular tracers have been horseradish peroxidase (HRP; don't use the cheapest grades, which contain toxic impurities; Sigma Type 6 is OK) and labelled plasma proteins. Rhodamine-conjugated bovine albumin is excellent. For HRP you need to cut frozen sections. Rhodamine-albumin is fine with paraffin. Another approach is simply to immunostain sections for endogenous plasma proteins.

John Kiernan
Anatomy, UWO
London, Canada.

----- Original Message -----
From: amritpal singh 
Date: Sunday, October 28, 2007 0:30
Subject: [Histonet] Help regarding staining of BBB

> Dear all, warm regards to you. I am here to find help from some 
> body who has experience in staining of blood brain barrier as it 
> is entirely a new area for my research group. We have heard of 
> India ink used to stain BBB, can any body provide me the 
> detailed procedure for it?
>   Looking for your kind guidance. I think you a lot in 
> anticipation for your cooperation.
>   Regards,
>   Amrit Pal Singh 
> ---------------------------------
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