A quick Google search for "BBB staining" brings up
over 400,000 hits.  I suspect a similar search on
PUBMED would bring up nearly as many (without some of
the dupicates and irrelevant hits in Google).  One of
the first papers I saw was from 1988 where they used
Evans Blue.  Most of the India Ink and Evans Blue
methods papers are from the 1950s and 1960s -- but I
don't know if there are any books with compilations of
the methods (there probably are--I just don't know
where).  EB is probably a better choice for light
microscopy, since it does label the endothelium.  Any
leakage of course will be evidence of damage to the
BBB.  I no longer have access to all my reprints and
reference manager, but this quick Google search shows
that there are more than one way to get answers.

Roger Moretz, Ph.D.
Electron Microscopist (retired)
"I'm not dead yet"  Monty Python, 'Search for the Holy
Grail'
--- amritpal singh  wrote:

> Dear all, warm regards to you. I am here to find
> help from some body who has experience in staining
> of blood brain barrier as it is entirely a new area
> for my research group. We have heard of India ink
> used to stain BBB, can any body provide me the
> detailed procedure for it?
>    
>   Looking for your kind guidance. I think you a lot
> in anticipation for your cooperation.
>    
>   Regards,
>   Amrit Pal Singh 
> 
>        
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