Re: [Histonet] H&E problem


Dear Michele Margiotta,
Just changing the first xylenes.

Muhammad Tahseen
Histology Supervisor,
Shaukat Khanum Memorial Cancer
Hospital & Research Centre

----- Original Message ----- 
From: "Margiotta, Michele" 
Sent: Wednesday, October 17, 2007 8:07 PM
Subject: [Histonet] H&E problem

Hi All,

We are having a problem with our H&E stain.  The pathologist is seeing 
circular areas on the tissues that are not staining with
the hematoxylin(Harris), but they are picking up the Eosin.  We are changing 
the first xylenes and alcohols and hoping that might help. Any idea why this 
might be happening?  Thanks!

Michele Margiotta
Histology Supervisor

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