Re: [Histonet] H&E problem

From:Rene J Buesa

Usually that artifact is due to incomplete dewaxing. Make sure your dewaxing reagent is fresh and the time adequate.
  René J.

"Margiotta, Michele"  wrote:
  Hi All,

We are having a problem with our H&E stain. The pathologist is seeing circular areas on the tissues that are not staining with
the hematoxylin(Harris), but they are picking up the Eosin. We are changing the first xylenes and alcohols and hoping that might help. Any idea why this might be happening? Thanks! 

Michele Margiotta
Histology Supervisor

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